Antimicrobial Susceptibility Testing Protocols

Antimicrobial Susceptibility Testing Protocols
edited by Richard Schwalbe

Antimicrobial Susceptibility Testing Protocols provides a comprehensive, up-to-date procedural manual that can be used by a wide variety of laboratory personnel. It includes protocols for bioassay methods and molecular methods for bacterial strain typing.

Antimicrobial Susceptibility Testing Protocols provides an integrated resource for updated lab-based techniques and charts within the contextual role of clinical microbiology in modern medicine.

Features:

• Utilizes a user-friendly, editorial style to explain tests and provide step-by-step instructions
• Offers clear procedural methods for use as a bench manual for laboratory personnel
• Explains procedures for standard and non-standard tests and protocols
• Describes the interaction between infectious disease physicians and laboratory personnel
• Includes a table of antibiotic classes and common bug-drug susceptibilities
• Offers extensive references at the end of each chapter to provide additional sources of information for investigating specific topics in greater detail

Part One introduces basic susceptibility disciplines including disk diffusion, macro and microbroth dilution, agar dilution, and the gradient method. It covers step-by-step protocols with an emphasis on optimizing the detection of resistant microorganisms.

Part Two describes specialized susceptibility protocols such as surveillance procedures for detection of antibiotic-resistant bacteria, serum bactericidal assays, time-kill curves, population analysis, and synergy testing.

Part Three is designed to be used as a reference resource. Chapters cover antibiotic development; design and use of an antibiogram; and the interactions of the clinical microbiology laboratory with the hospital pharmacy, and infectious disease and control.

Contents

1. An Overview of the Clinical and Laboratory Standards Institute and its Impact on Antimicrobial Susceptibility Testing
2. Antimicrobial Classifications: Drugs for Bugs
3. Disk Diffusion Tests and Gradient Methodologies
4. Macro and Microdilution Methods of Antimicrobial Susceptibility Testing
5. Automated Systems: An Overview
6. Agar Dilution Susceptibility Testing
7. Antibiograms/Preventive Surveillance: A Continuum of Data Collection, Analysis, and Presentation
8. Anaerobe Susceptibility Testing
9. Antifungal Susceptibility Testing of Yeasts
10. Antifungal Susceptibility Testing of Filamentous Fungi
11. Susceptibility Testing of Mycobacteria
12. Methods for Determining Bactericidal Activity and Antimicrobial Interactions: Synergy Testing/Time-Kill Curves/Population Analysis
13. Serum Bactericidal Testing
14. Bioassay Methods for Antimicrobial and Antifungal Agents
15. Molecular Methods for Bacterial Strain Typing
16. Pharmacy and Microbiology: Interactive Synergy
17. Interactions Between Clinicians and the Microbiology Laboratory
18. Clinical Microbiology in the Development of New Antimicrobial Agents

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